How to Choose an HPLC Column Supplier

 When choosing a column for HPLC analysis, there are several important things to look for. First, the column must be capable of the desired separation. The column should be able to separate proteins in the proper concentration range. This requires an effective coating. In some cases, a protein may interact with a stationary phase, and a coating that prevents this interaction may be necessary. In other cases, further method development may be necessary to achieve the desired separation. You may need to check this site chromtech.com to get a reliable columns supplier.

To determine whether your column is suitable for the separation that you require, you should determine the peak parameters. These include the peak resolution, the run time, and the loading capacity. You may also want to determine the compatibility of the column with the additives you intend to use. For example, if you need to separate a substance that exhibits a high level of tyrosine, you may want to choose a column with an As value of 0.7 to 1.6.

The lifetime of a column is another important factor. Typically, the longer the column, the better. For instance, a 15-cm column that operates at 11 Mpa backpressure will be capable of achieving a baseline separation for two species. However, the amount of reproducibility will depend on the separation goal.

In addition, the selection of a particle size for HPLC analysis is crucial. Smaller particles reduce the amount of time it takes a compound to migrate through a column. In addition, small particle size will reduce the number of interstitial voids that a compound pushes through the column.

Monolithic columns are made from a polymer or silica rod that is in contact with an acidic solution. These columns are very durable and require less maintenance. In addition, they are easy to clean. Some of the best-known manufacturers of HPLC columns are based on monolith technology. To get tried and tested HPLC columns, then get in touch with Chrom Tech.

There are many different types of HPLC columns, which range in size from capillary to process scale. The size of the column determines the amount of sample that can be loaded and the sensitivity of the separation. Typically, the smaller the column ID, the more sensitive the separation will be. In addition to size, the packing material should balance sensitivity and sample volume. Most analytical separations require silica packing, but polymeric resins are also available.

The size of the particle in an HPLC column can be influenced by the type of instrument used. For example, some analysts may wish to use a SEC column, which can be packed with a smaller particle size. In addition, the method should be easy to transfer from one HPLC to another. Check out this post that has expounded on the topic: https://en.wikipedia.org/wiki/Chromatography.