Choosing an HPLC Column Supplier
Chromatography supply houses have gained a lead over general laboratory distributors in recent years, mainly because of their stronger presence. However, the market share of general laboratory distributors has remained low, and only 18 percent of chromatographers buy their columns from them. General laboratory distributors are less technical than dedicated chromatography suppliers, and they tend to promote one-stop shopping. For your unique applications need, kindly view here; chromtech.com.
Columns should be tested to determine if they will work with your sample. Ensure that the column can tolerate the sample load, as this can affect SEC separation. Typically, a column should have a mass equivalent theoretical plate (HETP) value that remains constant when compared to the amount of sample loaded. For example, if you need to analyze PABA levels, then you should choose a column with a loading capacity (LC) of 20,000 or higher.
As column lifetimes continue to increase, users are still required to buy about seven new columns for each instrument. Some reasons for purchasing more columns include an increased volume of samples, or the procurement of improved columns. As a result, the HPLC column supplier should offer a variety of options that can meet your unique separation needs.
When choosing a column, it is important to understand the chemistry of the sample being separated. If you need to separate a monomer and a dimer, a short column may be the best choice. However, you must make sure that you are not exceeding the recommended maximum flow rate. You may need to experiment with different parameters, or refine your analytical process if necessary.
The ideal Chrom Tech HPLC column supplier will be able to provide you with a variety of options and customization. They can provide faster separations and higher sample throughput, as well as better resolution, lower pressure, and different types of selectivity. Additionally, they can adapt to your traditional HPLC system and run at higher pH.
HPLC columns are typically composed of high-purity particulate silica compressed into stainless steel tubing. Smaller particle sizes have shorter diffusion distances, which reduces the run times and increases the efficiency of the process. However, longer columns require higher pressures. Moreover, smaller particles also increase the backpressure.
The lifespan of a column is another critical parameter. Researchers recently surveyed HPLC users in Europe and North America to obtain a current profile of HPLC users. The surveys, which were last conducted in 2009, help chart trends in HPLC practice and column technology. To conduct their survey, LCGC published a web-based questionnaire in late 2010 to HPLC subscribers. 324 people responded, which was enough to make comparisons and investigate trends. Check out this post for more details related to this article: https://en.wikipedia.org/wiki/Column_chromatography.